Rapid and Variable-Volume Sampling Loading in Nonaqueous Microchip Electrophoresis
نویسندگان
چکیده
منابع مشابه
Nonaqueous Capillary Electrophoresis
answer. However, there is no reason why reactions cannot be carried out on such devices. Since it does not require the investment of a large chemical plant, the reactions can be performed where required, thus reducing the need to transport hazardous chemicals across countries. Since many reactors can be constructed on a single chip, and many chips located in the same area, it is evident that th...
متن کاملRapid microvolume PCR of DNA confirmed by microchip electrophoresis.
PCR is an indispensable technique used in DNA analysis. However, with the traditional methods, the time spent on amplification and the subsequent analysis of PCR products is generally long. Therefore, it is essential to improve these two steps so that the whole procedure can be made faster. In the present work, with lambda-DNA as the control template, the amplification of 300-bp fragment could ...
متن کاملRapid Biomolecule Analysis Using Two-dimensional Electrophoresis-electrospray Ionization Microchip
We demonstrate a two-dimensional (2D) separation microchip allowing capillary isoelectric focusing-capillary zone electrophoresis (cIEF-CZE) to be coupled to electrospray ionization mass spectrometry (ESI/MS). In the first dimension, separation is according to pI values (cIEF), while CZE occurs in the second dimension during mobilization towards the monolithically integrated ESI emitter. The se...
متن کاملRapid separation of microorganisms by quartz microchip capillary electrophoresis.
We developed and optimized a system coupling microchip capillary electrophoresis (MCE) and laser-induced fluorescence (LIF) detection for the analysis of microorganisms. The MCE-LIF system successfully separated pure cultures of lactic acid bacteria and Saccharomyces cerevisiae within 200 s. The results indicate that the MCE system can be conveniently used for the rapid and highly sensitive det...
متن کاملRapid on-column analysis of glucosamine and its mutarotation by microchip capillary electrophoresis.
A novel electrophoretic microchip method for analyzing alpha- and beta-d-glucosamine and their interconversion in solution is presented. d-Glucosamine is labeled with fluorescamine and analyzed by capillary electrophoresis in under 2 min revealing its pH-dependent mutarotation between the alpha- and beta-anomers. The forward interconversion rates for the labeled sugars, based on an iterative an...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of the Brazilian Chemical Society
سال: 2014
ISSN: 0103-5053
DOI: 10.5935/0103-5053.20140038